diamidino 2 phenylindole dapi staining (Servicebio Inc)
86
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Servicebio Inc
diamidino 2 phenylindole dapi staining
Diamidino 2 Phenylindole Dapi Staining, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+staining/2+4+6+diamidino+phenylindole/pmc13049910-67-22-29
Average 86 stars, based on 1 article reviews
Diamidino 2 Phenylindole Dapi Staining, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+staining/2+4+6+diamidino+phenylindole/pmc13049910-67-22-29
Average 86 stars, based on 1 article reviews
diamidino 2 phenylindole dapi staining - by Bioz Stars,
2026-09
86/100 stars
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Transfection:Article Title: CCL19-armed recombinant influenza virus inhibited colorectal cancer growth by remodeling tumor microenvironment. Article Snippet: The key instruments used in this study are as follows: Flow cytometer: Agilent NovoCyte Advanteon (1–3 Lasers) Flow Cytometer (Agilent Technologies, Santa Clara, CA, USA); Real-time cell analysis system: xCELLigence RTCA DP Instrument (Agilent Technologies, Santa Clara, CA, USA); Transmission electron microscope (TEM): Hitachi HT7800 Transmission Electron Microscope (Hitachi High-Technologies Corporation, Tokyo, Japan); Microplate reader: Molecular Devices SpectraMax iD5e Multimode Microplate Reader (Molecular Devices, San Jose, CA, USA); Gel imaging system: Thermo Fisher Scientific iBright CL1500 Imaging System (Catalog Number: 44114; Thermo Fisher Scientific, Waltham, MA, USA); Conventional PCR system: Bio-Rad T100 Thermal Cycler (Catalog Number: 1861096; Bio-Rad Laboratories, Hercules, CA, USA); Quantitative real-time PCR (qPCR) system: Bio-Rad CFX Connect Real-Time PCR Detection System (Catalog Number: 1855200; Bio-Rad Laboratories, Hercules, CA, USA); Nucleic acid electrophoresis system: Servicebio SVL-2 Integrated Horizontal Electrophoresis System (Servicebio Technology Co., Ltd., Wuhan, China); Vertical electrophoresis system: Servicebio SVE-4 Integrated Vertical Electrophoresis System (Servicebio Technology Co., Ltd., Wuhan, China); Centrifuge: Servicebio SLX-1024F High-Speed Refrigerated Centrifuge (Servicebio Technology Co., Ltd., Wuhan, China); Inverted microscope: Olympus IXplore IX85 Automated Inverted Microscope System (Olympus Corporation, Tokyo, Japan); Digital pathology scanner: 3D Histech Pannoramic SCAN Fully Automated Digital Pathology System (3D Histech Kft., Budapest, Hungary). .. Chemicals reagents The key chemical reagents used in this study are as follows: RPMI-1640 Medium, Gibco (Thermo Fisher Scientific, Waltham, MA, USA), Catalog Number: C22400500BT; RPMI-DMEM Medium, Gibco (Thermo Fisher Scientific, Waltham, MA, USA), Catalog Number: C11995500BT; Fetal Bovine Serum (FBS), Newzerum (Newzerum Biotechnology Co., Ltd., China), Catalog Number: FBSPA500; Polyfect Transfection Reagent, Qiagen (Qiagen N.V., Hilden, Germany), Catalog Number: 301105; 2×Taq Mix, Vazyme (Vazyme Biotech Co., Ltd., Nanjing, China), Catalog Number: P213-01/02/03; Matrigel, Shanghai Nova (Shanghai Nova Biotechnology Co., Ltd., Shanghai, China), Catalog Number: Article Title: CCL19-armed recombinant influenza virus inhibited colorectal cancer growth by remodeling tumor microenvironment Article Snippet: The key instruments used in this study are as follows: Flow cytometer: Agilent NovoCyte Advanteon (1–3 Lasers) Flow Cytometer (Agilent Technologies, Santa Clara, CA, USA); Real-time cell analysis system: xCELLigence RTCA DP Instrument (Agilent Technologies, Santa Clara, CA, USA); Transmission electron microscope (TEM): Hitachi HT7800 Transmission Electron Microscope (Hitachi High-Technologies Corporation, Tokyo, Japan); Microplate reader: Molecular Devices SpectraMax iD5e Multimode Microplate Reader (Molecular Devices, San Jose, CA, USA); Gel imaging system: Thermo Fisher Scientific iBright CL1500 Imaging System (Catalog Number: 44114; Thermo Fisher Scientific, Waltham, MA, USA); Conventional PCR system: Bio-Rad T100 Thermal Cycler (Catalog Number: 1861096; Bio-Rad Laboratories, Hercules, CA, USA); Quantitative real-time PCR (qPCR) system: Bio-Rad CFX Connect Real-Time PCR Detection System (Catalog Number: 1855200; Bio-Rad Laboratories, Hercules, CA, USA); Nucleic acid electrophoresis system: Servicebio SVL-2 Integrated Horizontal Electrophoresis System (Servicebio Technology Co., Ltd., Wuhan, China); Vertical electrophoresis system: Servicebio SVE-4 Integrated Vertical Electrophoresis System (Servicebio Technology Co., Ltd., Wuhan, China); Centrifuge: Servicebio SLX-1024F High-Speed Refrigerated Centrifuge (Servicebio Technology Co., Ltd., Wuhan, China); Inverted microscope: Olympus IXplore IX85 Automated Inverted Microscope System (Olympus Corporation, Tokyo, Japan); Digital pathology scanner: 3D Histech Pannoramic SCAN Fully Automated Digital Pathology System (3D Histech Kft., Budapest, Hungary). .. The key chemical reagents used in this study are as follows: RPMI-1640 Medium, Gibco (Thermo Fisher Scientific, Waltham, MA, USA), Catalog Number: C22400500BT; RPMI-DMEM Medium, Gibco (Thermo Fisher Scientific, Waltham, MA, USA), Catalog Number: C11995500BT; Fetal Bovine Serum (FBS), Newzerum (Newzerum Biotechnology Co., Ltd., China), Catalog Number: FBS-PA500; Polyfect Transfection Reagent, Qiagen (Qiagen N.V., Hilden, Germany), Catalog Number: 301105; 2×Taq Mix, Vazyme (Vazyme Biotech Co., Ltd., Nanjing, China), Catalog Number: P213-01/02/03; Matrigel, Shanghai Nova (Shanghai Nova Biotechnology Co., Ltd., Shanghai, China), Catalog Number: Staining:Article Title: CCL19-armed recombinant influenza virus inhibited colorectal cancer growth by remodeling tumor microenvironment. Article Snippet: The key instruments used in this study are as follows: Flow cytometer: Agilent NovoCyte Advanteon (1–3 Lasers) Flow Cytometer (Agilent Technologies, Santa Clara, CA, USA); Real-time cell analysis system: xCELLigence RTCA DP Instrument (Agilent Technologies, Santa Clara, CA, USA); Transmission electron microscope (TEM): Hitachi HT7800 Transmission Electron Microscope (Hitachi High-Technologies Corporation, Tokyo, Japan); Microplate reader: Molecular Devices SpectraMax iD5e Multimode Microplate Reader (Molecular Devices, San Jose, CA, USA); Gel imaging system: Thermo Fisher Scientific iBright CL1500 Imaging System (Catalog Number: 44114; Thermo Fisher Scientific, Waltham, MA, USA); Conventional PCR system: Bio-Rad T100 Thermal Cycler (Catalog Number: 1861096; Bio-Rad Laboratories, Hercules, CA, USA); Quantitative real-time PCR (qPCR) system: Bio-Rad CFX Connect Real-Time PCR Detection System (Catalog Number: 1855200; Bio-Rad Laboratories, Hercules, CA, USA); Nucleic acid electrophoresis system: Servicebio SVL-2 Integrated Horizontal Electrophoresis System (Servicebio Technology Co., Ltd., Wuhan, China); Vertical electrophoresis system: Servicebio SVE-4 Integrated Vertical Electrophoresis System (Servicebio Technology Co., Ltd., Wuhan, China); Centrifuge: Servicebio SLX-1024F High-Speed Refrigerated Centrifuge (Servicebio Technology Co., Ltd., Wuhan, China); Inverted microscope: Olympus IXplore IX85 Automated Inverted Microscope System (Olympus Corporation, Tokyo, Japan); Digital pathology scanner: 3D Histech Pannoramic SCAN Fully Automated Digital Pathology System (3D Histech Kft., Budapest, Hungary). .. Chemicals reagents The key chemical reagents used in this study are as follows: RPMI-1640 Medium, Gibco (Thermo Fisher Scientific, Waltham, MA, USA), Catalog Number: C22400500BT; RPMI-DMEM Medium, Gibco (Thermo Fisher Scientific, Waltham, MA, USA), Catalog Number: C11995500BT; Fetal Bovine Serum (FBS), Newzerum (Newzerum Biotechnology Co., Ltd., China), Catalog Number: FBSPA500; Polyfect Transfection Reagent, Qiagen (Qiagen N.V., Hilden, Germany), Catalog Number: 301105; 2×Taq Mix, Vazyme (Vazyme Biotech Co., Ltd., Nanjing, China), Catalog Number: P213-01/02/03; Matrigel, Shanghai Nova (Shanghai Nova Biotechnology Co., Ltd., Shanghai, China), Catalog Number: Article Title: Hemozoin induces neuronal injury primarily characterized by axon rupture and mitochondrial damage in experimental cerebral malaria. Article Snippet: .. Coronal sections (5 μm) underwent heat-induced epitope retrieval in citrate buffer, followed by 3% H2O2 treatment, blocking with 3% BSA/2% bovine serum/0.2% Triton X-100 in PBS for 30 min, primary antibody incubation at 4°C overnight, secondary AR TIC LE IN PR ES S antibody incubation at room temperature for 1 h, and Article Title: Co-administration of berberine and evodiamine: Mitigating evodiamine-induced hepatotoxicity and potentiating colitis treatment. Article Snippet: Background: The combined therapeutic mechanisms of evodiamine (EVO) and berberine (BBR), which are the primary bioactive components contributing to the pharmacological effects of Euodiae Fructus and Rhizoma Coptidis, a classic herb pair used for colitis, have not yet been fully elucidated Purpose: This study aimed to investigate the critical mechanisms by which co-administration EVO and BBR mitigated EVO-induced hepatotoxicity while exerting therapeutic effects against colitis.. Methods: Safety and therapeutic outcomes of EVO alone and combined BBR were validated in normal and colitis models.. RNA-seq sequencing was performed on liver and colon tissues from different groups, while metagenomic sequencing was utilized to analysis fecal samples. Article Title: Hemozoin induces neuronal injury primarily characterized by axon rupture and mitochondrial damage in experimental cerebral malaria Article Snippet: .. Coronal sections (5 μm) underwent heat-induced epitope retrieval in citrate buffer, followed by 3% H2O2 treatment, blocking with 3% BSA/2% bovine serum/0.2% Triton X-100 in PBS for 30 min, primary antibody incubation at 4 °C overnight, secondary antibody incubation at room temperature for 1 h, and Article Title: Polydopamine/chitosan layer-by-layer coating of Bacillus cereus enhances the robustness of live anti-oxidation materials for the treatment of radiation enteritis Article Snippet: Low viscosity chitosan, dopamine hydrochloride, trihydroxymethylaminomethane hydrochloride, 3-hydroxymethylaminomethane, and rhodamine B were purchased from Beijing Innochem Technology Co., Ltd. (Beijing, China). .. Article Title: CD142-positive synovial fibroblasts drive meniscus destruction in rheumatoid arthritis. Article Snippet: IF440-TSA (anti-CD146, Servicebio, 1:500), iF488-TSA (anti-CD142, Servicebio, 1:500), iF555-TSA (anti-PRG4, Servicebio, 1:500) and iF647-TSA (anti-THY1, Servicebio, 1:500) were used for TSA staining. .. Article Title: CCL19-armed recombinant influenza virus inhibited colorectal cancer growth by remodeling tumor microenvironment Article Snippet: The key instruments used in this study are as follows: Flow cytometer: Agilent NovoCyte Advanteon (1–3 Lasers) Flow Cytometer (Agilent Technologies, Santa Clara, CA, USA); Real-time cell analysis system: xCELLigence RTCA DP Instrument (Agilent Technologies, Santa Clara, CA, USA); Transmission electron microscope (TEM): Hitachi HT7800 Transmission Electron Microscope (Hitachi High-Technologies Corporation, Tokyo, Japan); Microplate reader: Molecular Devices SpectraMax iD5e Multimode Microplate Reader (Molecular Devices, San Jose, CA, USA); Gel imaging system: Thermo Fisher Scientific iBright CL1500 Imaging System (Catalog Number: 44114; Thermo Fisher Scientific, Waltham, MA, USA); Conventional PCR system: Bio-Rad T100 Thermal Cycler (Catalog Number: 1861096; Bio-Rad Laboratories, Hercules, CA, USA); Quantitative real-time PCR (qPCR) system: Bio-Rad CFX Connect Real-Time PCR Detection System (Catalog Number: 1855200; Bio-Rad Laboratories, Hercules, CA, USA); Nucleic acid electrophoresis system: Servicebio SVL-2 Integrated Horizontal Electrophoresis System (Servicebio Technology Co., Ltd., Wuhan, China); Vertical electrophoresis system: Servicebio SVE-4 Integrated Vertical Electrophoresis System (Servicebio Technology Co., Ltd., Wuhan, China); Centrifuge: Servicebio SLX-1024F High-Speed Refrigerated Centrifuge (Servicebio Technology Co., Ltd., Wuhan, China); Inverted microscope: Olympus IXplore IX85 Automated Inverted Microscope System (Olympus Corporation, Tokyo, Japan); Digital pathology scanner: 3D Histech Pannoramic SCAN Fully Automated Digital Pathology System (3D Histech Kft., Budapest, Hungary). .. The key chemical reagents used in this study are as follows: RPMI-1640 Medium, Gibco (Thermo Fisher Scientific, Waltham, MA, USA), Catalog Number: C22400500BT; RPMI-DMEM Medium, Gibco (Thermo Fisher Scientific, Waltham, MA, USA), Catalog Number: C11995500BT; Fetal Bovine Serum (FBS), Newzerum (Newzerum Biotechnology Co., Ltd., China), Catalog Number: FBS-PA500; Polyfect Transfection Reagent, Qiagen (Qiagen N.V., Hilden, Germany), Catalog Number: 301105; 2×Taq Mix, Vazyme (Vazyme Biotech Co., Ltd., Nanjing, China), Catalog Number: P213-01/02/03; Matrigel, Shanghai Nova (Shanghai Nova Biotechnology Co., Ltd., Shanghai, China), Catalog Number: Blocking Assay:Article Title: Hemozoin induces neuronal injury primarily characterized by axon rupture and mitochondrial damage in experimental cerebral malaria. Article Snippet: .. Coronal sections (5 μm) underwent heat-induced epitope retrieval in citrate buffer, followed by 3% H2O2 treatment, blocking with 3% BSA/2% bovine serum/0.2% Triton X-100 in PBS for 30 min, primary antibody incubation at 4°C overnight, secondary AR TIC LE IN PR ES S antibody incubation at room temperature for 1 h, and Article Title: Hemozoin induces neuronal injury primarily characterized by axon rupture and mitochondrial damage in experimental cerebral malaria Article Snippet: .. Coronal sections (5 μm) underwent heat-induced epitope retrieval in citrate buffer, followed by 3% H2O2 treatment, blocking with 3% BSA/2% bovine serum/0.2% Triton X-100 in PBS for 30 min, primary antibody incubation at 4 °C overnight, secondary antibody incubation at room temperature for 1 h, and Incubation:Article Title: Hemozoin induces neuronal injury primarily characterized by axon rupture and mitochondrial damage in experimental cerebral malaria. Article Snippet: .. Coronal sections (5 μm) underwent heat-induced epitope retrieval in citrate buffer, followed by 3% H2O2 treatment, blocking with 3% BSA/2% bovine serum/0.2% Triton X-100 in PBS for 30 min, primary antibody incubation at 4°C overnight, secondary AR TIC LE IN PR ES S antibody incubation at room temperature for 1 h, and Article Title: Hemozoin induces neuronal injury primarily characterized by axon rupture and mitochondrial damage in experimental cerebral malaria Article Snippet: .. Coronal sections (5 μm) underwent heat-induced epitope retrieval in citrate buffer, followed by 3% H2O2 treatment, blocking with 3% BSA/2% bovine serum/0.2% Triton X-100 in PBS for 30 min, primary antibody incubation at 4 °C overnight, secondary antibody incubation at room temperature for 1 h, and Cell Culture:Article Title: Hemozoin induces neuronal injury primarily characterized by axon rupture and mitochondrial damage in experimental cerebral malaria. Article Snippet: .. Coronal sections (5 μm) underwent heat-induced epitope retrieval in citrate buffer, followed by 3% H2O2 treatment, blocking with 3% BSA/2% bovine serum/0.2% Triton X-100 in PBS for 30 min, primary antibody incubation at 4°C overnight, secondary AR TIC LE IN PR ES S antibody incubation at room temperature for 1 h, and Article Title: Hemozoin induces neuronal injury primarily characterized by axon rupture and mitochondrial damage in experimental cerebral malaria Article Snippet: .. Coronal sections (5 μm) underwent heat-induced epitope retrieval in citrate buffer, followed by 3% H2O2 treatment, blocking with 3% BSA/2% bovine serum/0.2% Triton X-100 in PBS for 30 min, primary antibody incubation at 4 °C overnight, secondary antibody incubation at room temperature for 1 h, and AST Assay:Article Title: Co-administration of berberine and evodiamine: Mitigating evodiamine-induced hepatotoxicity and potentiating colitis treatment. Article Snippet: Background: The combined therapeutic mechanisms of evodiamine (EVO) and berberine (BBR), which are the primary bioactive components contributing to the pharmacological effects of Euodiae Fructus and Rhizoma Coptidis, a classic herb pair used for colitis, have not yet been fully elucidated Purpose: This study aimed to investigate the critical mechanisms by which co-administration EVO and BBR mitigated EVO-induced hepatotoxicity while exerting therapeutic effects against colitis.. Methods: Safety and therapeutic outcomes of EVO alone and combined BBR were validated in normal and colitis models.. RNA-seq sequencing was performed on liver and colon tissues from different groups, while metagenomic sequencing was utilized to analysis fecal samples. Malondialdehyde MDA Assay:Article Title: Co-administration of berberine and evodiamine: Mitigating evodiamine-induced hepatotoxicity and potentiating colitis treatment. Article Snippet: Background: The combined therapeutic mechanisms of evodiamine (EVO) and berberine (BBR), which are the primary bioactive components contributing to the pharmacological effects of Euodiae Fructus and Rhizoma Coptidis, a classic herb pair used for colitis, have not yet been fully elucidated Purpose: This study aimed to investigate the critical mechanisms by which co-administration EVO and BBR mitigated EVO-induced hepatotoxicity while exerting therapeutic effects against colitis.. Methods: Safety and therapeutic outcomes of EVO alone and combined BBR were validated in normal and colitis models.. RNA-seq sequencing was performed on liver and colon tissues from different groups, while metagenomic sequencing was utilized to analysis fecal samples. Enzyme-linked Immunosorbent Assay:Article Title: Co-administration of berberine and evodiamine: Mitigating evodiamine-induced hepatotoxicity and potentiating colitis treatment. Article Snippet: Background: The combined therapeutic mechanisms of evodiamine (EVO) and berberine (BBR), which are the primary bioactive components contributing to the pharmacological effects of Euodiae Fructus and Rhizoma Coptidis, a classic herb pair used for colitis, have not yet been fully elucidated Purpose: This study aimed to investigate the critical mechanisms by which co-administration EVO and BBR mitigated EVO-induced hepatotoxicity while exerting therapeutic effects against colitis.. Methods: Safety and therapeutic outcomes of EVO alone and combined BBR were validated in normal and colitis models.. RNA-seq sequencing was performed on liver and colon tissues from different groups, while metagenomic sequencing was utilized to analysis fecal samples. CCK-8 Assay:Article Title: Co-administration of berberine and evodiamine: Mitigating evodiamine-induced hepatotoxicity and potentiating colitis treatment. Article Snippet: Background: The combined therapeutic mechanisms of evodiamine (EVO) and berberine (BBR), which are the primary bioactive components contributing to the pharmacological effects of Euodiae Fructus and Rhizoma Coptidis, a classic herb pair used for colitis, have not yet been fully elucidated Purpose: This study aimed to investigate the critical mechanisms by which co-administration EVO and BBR mitigated EVO-induced hepatotoxicity while exerting therapeutic effects against colitis.. Methods: Safety and therapeutic outcomes of EVO alone and combined BBR were validated in normal and colitis models.. RNA-seq sequencing was performed on liver and colon tissues from different groups, while metagenomic sequencing was utilized to analysis fecal samples. |
